Not known Facts About attune flow cytometer
Not known Facts About attune flow cytometer
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Details-enriched characterization of cell populations. Morphological facts from photographs can increase to the richness of flow cytometry info. As an example, the determine shows an if not common apoptosis assay employing Annexin V and PI, including mobile imaging to characterize cells in Every population to expose morphologically unique options. These insights could not are actually obtained from multiplex staining by itself.
As samples are acquired over the Attune CytPix Flow Cytometer, the high-pace brightfield camera captures and suppliers photographs of detected activities at a price approximately 6,000 pictures for each second, dependant upon the flow rate and image dimensions. For better versatility, Attune Cytometric Program helps you to regulate the picture capture frequency as essential and select a certain gate for picture seize, if expected.
Blend acoustic focusing technological innovation with high-parameter Examination for adaptable spectral unmixing and conventional workflows.
The award-successful Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing by using a higher-pace brightfield digicam for simultaneous high throughput flow cytometry and higher resolution brightfield imaging.
*Pricing is indicative only and is topic to change. Please Get in touch with your Agilent representative with any concerns. The data established forth herein is not an offer or even a commitment, equally of which might be topic to credit rating approval, conditions and terms, and availability with Agilent. Agilent Economic Solutions is obtainable in chosen countries.
Here, a seasoned consumer has gated singlets confidently. Immediately after analyzing the handbook singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate incorporates greater than four% aggregates.
Optimizing gating system. Even strong handbook singlet gating is mistake-susceptible and stays a subjective decision level in Pretty much all flow cytometry assays. Imaging can be utilized to verify and regulate gates to include only solitary cells of curiosity.
Reliable picture high quality even at large throughput Acoustic concentrating and also a significant-velocity digicam Mix to graphic these T cells consistently at low or high flow charges. Quickly regulate concentrate and camera settings to fulfill experimental prerequisites.
Early indications of this contamination had in the beginning been dismissed as debris. Backgating to find out new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also permits you to use morphological attributes to discover fascinating subpopulations that may not be clear from flow cytometry knowledge alone.
Most likely most significantly, these occasions comprise cells of clearly distinct phenotypes which could lead on to incorrect conclusions pertaining to double positive situations (especially in rare populations).
AI-pushed automated impression analysis Along with the Attune Cytometric Software package translates occasion functions into unique morphology parameters that could be combined with normal fluorescence and scatter parameters to expose extremely reproducible inhabitants changes and special subsets.
Software Be aware: Automatic graphic Assessment lowers person-to-person variability in flow cytometry gating strategies
Built-in dimensionality reduction resources improved resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to confirm anticipated population resolution in lysed total human blood. User can view situations from Just about every cluster for visual confirmation of mobile phenotypes.
Flexible payment system from Agilent Economic Answers allow you to have the essential instrumentation you'll need for quick venture, or to mature your lab.
Gating on these extended parameters means that you can speedily and correctly identify populations of curiosity and confirm gating tactic with little if any guide evaluation. The Attune software program features entry to state-of-the-art machine Understanding Assessment applications within just an expertly designed consumer interface, making it exceptionally simple to operate.
The Attune Xenith cytometer is really a superior-parameter spectral analyzer, giving equally spectral unmixing and conventional flow capabilities for extensive and multipurpose cell Investigation.
The Attune CytPix Flow here Cytometer permits buyers to visualize samples during acquisition, validate the precision of gating tactics, and support outcomes with reputable documentation for publication uses.
What gave the impression to be a homogeneous population is really far more diverse—an Perception that needs to be thought of when interpreting final results.